Original ArticleThe RPS Adeno Detector for Diagnosing Adenoviral Conjunctivitis
Section snippets
Description of the RPS Adeno Detector
The RPS Adeno Detector is based on the principle of lateral flow immunochromatography. Two antigen-specific antibodies capture viral antigens or particles. The RPS Adeno Detector detects common epitopes on the hexon protein of the adenovirus within a conserved region among the serotypes.27, 28, 29, 30, 31, 32, 33, 34 This allows the RPS Adeno Detector to detect all known serotypes. One antibody is immobilized in the detection zone of the device. The second antibody is labeled with colloidal
Results
A total of 186 consecutive patients with acute conjunctivitis were tested at 5 clinical sites. Study patients were 64.5% (n = 120) female and 35.5% (n = 66) male, and the age ranged from 1 to 84 years of age, with a mean age of 41.8 years. Adenovirus was confirmed by PCR in 25.3% of all patients with a red eye at presentation.
In reference to cell culture (CC-IFA), the RPS Adeno Detector demonstrated a sensitivity of 37 of 42 (88%) and a specificity of 121 of 133 (91%), and an overall agreement
Discussion
Historically, the true gold standard for adenovirus conjunctivitis diagnosis is cell culture (CC-IFA). For many physicians, PCR now is considered the new gold standard. Culture has many potential shortcomings because it relies on an adequate collection of viable virus, survival of the virus during transport, growth of the virus on the medium, and recognition of the cytopathic effect by the technician.20 Newer PCR techniques, however, are able to amplify small amounts of viral DNA and therefore
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Manuscript no. 2006-119.
Rapid Pathogen Screening, Inc., South Williamsport, Pennsylvania, paid for all laboratory studies.
None of the principal investigators has any financial interest.
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Dr Sambursky is the medical director and a shareholder of Rapid Pathogen Screening.